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Published on:2, 2011
Pharmacognosy Journal, 2011; 3(20):66-71
Original Article | doi:10.5530/pj.2011.20.13

Evaluation of Brine shrimp lethality and Antimicrobial activity of Azadirachta indica leaf extract on some drug resistance bacteria in Bangladesh

Authors and affiliation (s):

Abdullah-Al-Emran1, S.M Shahed1, Farzana Ahmed1, Sajal Kumar Saha2, Sreedam Chandra Das3, Sitesh Chandra Bachar4

1Department of Genetic Engineering and Biotechnology, University of Dhaka, Bangladesh,

2Department of Clinical Pharmacy and Pharmacology, University of Dhaka, Bangladesh,

3Department of Pharmacy, Southeast University, Dhaka, Bangladesh.

4Department of Pharmaceutical Technology, University of Dhaka.


Azadirachta indica (Neem) is very useful traditional medicinal plant in the subcontinent. Each part of the tree has some medicinal properties. In this present studies the bioactivity and antimicrobial activity of Azadirachta indica leaf extract was evaluated. For bioactivity, Brine shrimp lethality test was done for A. indica ethanolic leaf extract and determined LC50 was 37.15 mg/ml indicating that the prepared extract was rich in bioactive compounds. Ethanolic leaf extract was also used to evaluate antibacterial activity and the extract exhibited significant activity against the tested bacterial strain. However, inhibitory activity was concentration and test organism dependent. Gram-positive bacteria were more sensitive to the extract compare to Gram-negative bacteria. All of the tested bacteria showed sensitivity at higher concentration (7mg/ml) but multi drug resistant bacteria Klebsiella pneumoniae was very sensitive even at very low concentration (2mg/ml). Further, the results depict that leaf extracts of Azadirachta indica could be used as a potential source of antimicrobial agents against the bacterial strains tested.

Key words: Azadirachta indica, Medicinal Plant, Antibacterial Activity, Agar well diffusion assay.


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