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Published on:1, 2011
Pharmacognosy Journal, 2011; 3(19):57-64
Original Article | doi:10.5530/pj.2011.19.11

Cyclooxygenase Inhibitory, Cytotoxicity and Free Radical Scavenging Activities of Selected Medicinal Plants Used in Indian Traditional Medicine


Authors and affiliation (s):

Rajesh Gacche*, Rafik Shaikh, Mahesh Pund, Rupesh Deshmukh

School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded - 431 606 (MS), India.

Abstract:

Introduction: The enzyme cyclooxygenase (COX) has been implicated to be a key enzyme involved in recruiting inflammations. Developing COX inhibitors has remained one of the important aspects of developing novel and safe antiinflammatory agents. An attempt has made to find the medicinal plants as alternatives to presently available NSAIDs (Non steroidal anti-inflammatory drugs). Methods: In the present study the samples of Enicostema axillare (Lam.) Raynal. [Gentianaceae], Argemone mexicana L. [Papaveraceae], Clerodendrum multiflorum (Burm.f.) O. Ktze. [Verbenaceae], Withania somnifera (L.) Dunal. [Solanaceae], Polyalthia longifolia (Sonner.) Thw. [Annonaceae] and Vitex nigundo L. [Verbenaceae] were sequentially extracted in water, ethanol and hexane and were evaluated in-vitro for COX-1 and 2 inhibitory activities. The free radical scavenging activities were carried out along with cytotoxicity evaluation. Results: Among the tested plants, E. axillare showed promising COX-2 inhibiting activity in ethanol (48.71 ± 0.035 %), water (42.13 ± 0.030 %) and hexane (12.31 ± 0.040 %) as compared to COX-1 inhibition in ethanol (14.73 ± 0.030 %), water (27.64 ± 0.030 %) and hexane (6.92 ± 0.031 %). The contents of the water extracts of majority of the plant samples were found to interact with DPPH, superoxide and OH radicals. The selected plants did not showed cytoxicity except a poor toxicity demonstrated by ethanol extract of P. longifolia (0.15 ± 0.040 %). HPTLC analysis was carried out to study the flavonoids diversity of the selected plant samples. Conclusion: The results obtained shows that majority of the plants under study were found to inhibit COX-2 activity significantly as compared to COX-1 activity. However, more detailed studies are required to assess the safety and efficacy of these plants.

Key words: Anti-inflammatory, Cyclooxygenase (COX), Medicinal plants, Antioxidants, Cytotoxicity.

 

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