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Published on:26th May 2014
Pharmacognosy Journal, 2014; 6(4):10-15
Research Article | doi:10.5530/pj.2014.4.2

Isolation and identification of endophytic fungi in the medicinal plant Mikania laevigata (Asteraceae)

Authors and affiliation (s):

Fernanda P.C. Ribeiroª, Hélio M Kamidab, Paulo R.M. Almeidab, Ana P.T. Uetanabaroc, Larissa C.B. Costac and Alexsandro Brancoª

ªLaboratório de Fitoquímica, Departamento de Saúde, Universidade Estadual de Feira de Santana, Campus Universitário, Av. Transnordestina, s/nº, Novo Horizonte, 44.036–900 Feira de Santana, BA, Brazil

bLaboratório de Pesquisa em Microbiologia, Departamento de Biologia, Universidade Estadual de Feira de Santana,Campus Universitário, Av. Transnordestina, s/nº, Bairro Novo Horizonte, 44.036–900 Feira de Santana, BA, Brazil

cDepartamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, 45.662–900 Ilheus, BA,Brazil.


The Mikania laevigata (Asteraceae) is a Brazilian medicinal plant used to treat respiratory tract diseases.It is produced on a large scale for phytotherapic formulation. No studies on the endophytes of Mikania laevigata have been described in the literature. The endophytic fungi isolated from the leaves of M. laevigata cultivated in a floral garden located in the south region of the Bahia state, Brazil, were investigated. After surface disinfection, the performing the isolation procedure, a total of forty endophytic fungi were obtained and grouped into different morphospecies. The fungi were characterized by molecular sequencing the ITS rDNA regions, and they were identified by comparing the results published Gen Bank sequences.The phylogenetic analyses revealed four major clades of fungi from the sequenced ITS-rDNA regions: Nodulisporium sp. (3 isolates), Hypoxylon sp.(3 isolates), Daldinia sp. (1isolate) and Xylaria luteo stromata (aunique isolate).The Xylariaceae Genus was the dominant group of fungi associated with Mikania laevigata.

Key words:  Mikania laevigata, Asteraceae, Xylariaceae, Endophytic fungi.


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