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Published on:10, 2011
Pharmacognosy Journal, 2011; 3(26):54-60
Original Article | doi:10.5530/pj.2011.26.10

Phytochemical Screening and in vitro Antioxidant Activity of Jawarish Amla- A Poly Herbal Formulation

Authors and affiliation (s):

Mohd Amir1, Ahsanullah Khan1, Mohd Mujeeb1*, Mohd Aftab Ahmad2, Nadeem Ahmad Siddiqui1

1 Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi-62, India.

2Department of ilmul advia, Faculty of medicine (unani), Jamia Hamdard, New Delhi-62, India.


The present research work was carried out to study the antioxidant potential of methanolic extract of Jawarish amla using various in vitro tests including Reducing power assay, Superoxide anion scavenging activity assay, Hydroxyl radical scavenging activity assay, Nitric oxide scavenging activity assay, DPPH free radical scavenging assay, and hydrogen peroxide method. These various antioxidant activities were compared to standard ascorbic acid. Preliminary phytochemical screening revealed that the extract of jawarish amla possesses flavonoids, steroids and phenolic compounds. In this study, quantitative determinations of flavonoids and phenols were conducted by colorimetric methods, using aluminum chloride method and Folin Ciocalteu reagent respectively. The results indicated that methanolic extract of Jawarish amla possess antioxidant property.

Key words: Antioxidant activity, Ascorbic acid, DPPH, Jawarish Amla.


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