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Published on:10, 2011
Pharmacognosy Journal, 2011; 3(26):61-69
Original Article | doi:10.5530/pj.2011.26.11

Phytochemical Investigation of Methanolic Extract of Cassia fistula Leaves

Authors and affiliation (s):

Manisha A. Nagpal1* Navneet Nagpal2, Sandeep Rahar1, Gagan Shah1, Gaurav Swami3, Reni Kapoor4

1Department of Pharmaceutical Chemistry, B.I.S. College of Pharmacy, Gagra, Moga, India,

2Department of Pharmaceutics, Khalsa College of Pharmacy, Amritsar, India,

3Department of Pharmaceutics, C.T. Institute of Technology, Jalandhar, India.

4Department of Pharmacognosy, Akal College of Pharmacy, Masstuana Sahib, Sangrur, India.


Introduction: Recently there has been a shift in universal trend from synthetic to herbal medicine, which we can say’ Return to Nature’. Cassia fistula or golden shower plant has plenty of medicinal values. This paper represent the isolation of two new chemical entities, present the leaves of cassia fistula and their medicinal aspects. Methods: Leaves of Cassia fistula was used as a plant material. Extraction was done with methanol followed by isolation procedure was done using HPTLC. Confirmation of compounds was done using UV, FTIR, NMR and MASS spectroscopy. In vitro antibacterial screening was carried out by disc diffusion method. Results: Air dried powdered leaves of Cassia fistula were extracted using methanol. From this extract two compounds were isolated in their pure form using Column chromatography and HPTLC. Identification of these two isolated compounds was confirmed by physico-chemical data, spectral interpretation and elemental analysis. Conclusions: This is the first report of the isolation of the 2 new compounds from cassia fistula leaves extracted in methanol extract. These compounds are seen to exhibit moderate antimicrobial property.

Key words: Antimicrobial activity, HPTLC, MASS, NMR.


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