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Published on:11th Aug, 2012
Pharmacognosy Journal, 2012; 4(30):20-24
Original Article | doi:10.5530/pj.2012.30.4

Antidiabetic Activity of Standardized Extracts of Balanites aegyptiaca Fruits using Cell-based Bioassays


Authors and affiliation (s):

Amira Abdel Motaal,1* Sherif Shaker,2 Pierre S. Haddad3

1Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy St., Cairo 11562, Egypt

2Pharmaceutical Department, Heliopolis Academy, Sekem, 3 Cairo-Belbeis Road, El Horreya, Cairo, Egypt, P.B. 2834.

3Natural Health Products and Metabolic Diseases Laboratory, Department of Pharmacology and Montreal Diabetes Research Centre, Université de Montréal, Montreal, Quebec, Canada H3 C3J7.

Abstract:

The antidiabetic activities of different extracts and fractions of Balanites aegyptiaca were tested in cultured C2C12 skeletal muscle cells and 3T3-L1 adipocytes. An 18-h treatment with 200 μg/mL of the sugars fraction (A1) showed the highest activity as it increased basal glucose uptake by 52% in muscle cells; which is twice the activity of 100 nM insulin [insulin equivalent (IE) = 2.0 ± 0.07]. The dichloromethane (E) and ethyl acetate (F) successive extracts exerted 37 and 41% increase in the glucose uptake, respectively. Only E and F accelerated the triglyceride accumulation in pre-adipocytes undergoing differentiation, comparably with 10 μM rosiglitazone [rosiglitazone equivalent (RE) was 1.6 ± 0.3 and 0.7 ± 0.1, respectively). Gas chromatography (GC) analysis of A1 revealed the presence of xylose, rhamnose, sorbitol, fructose, galactose and glucose. The active extracts E and F were standardized by high-performance liquid chromatography (HPLC) to contain 0.031 and 0.239% of rutin, 0.007 and 0.004% of isorhamnetin, respectively.

Key words: balanites, C2C12, diabetes, fruit, sugars, 3T3‑L1.

 

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