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Published on:3rd Sept, 2014
Pharmacognosy Journal, 2014; 6(6):32-38
Original Article | doi:10.5530/pj.2014.6.7

Antiproliferative activity of crude extract and fractions obtained from Digera muricata on Hela cell lines of human cervix and A549 cell lines of Human Lung.


Authors and affiliation (s):

Shazia Usmania*, Arshad Hussaina, A.H.A Farooquib, Mohd.Arshadc, Sahabjada Siddiquic, Mohd.Ahmada, Shadma Wahaba

aFaculty of Pharmacy, Integral University, Kursi Road, Lucknow-226026, INDIA.

bFaculty of Biosciences, Integral University, Kursi Road, Lucknow-226026, INDIA.

cDepartment of Zoology, Faculty of Life Sciences, Lucknow University, Lucknow, INDIA.

Abstract:

Digera muricata (Linn.) of family Amaranthaceae is an ethanobotanically important plant species traditionally used against various disorders. Cytotoxic potential of methanolic extract and its fractions were investigated against Hela and A549 cell lines. Crude extract of Digera muricata was prepared in methanol by Continuous Hot Soxhlation technique. Crude extract was fractionated into two organic and one aqueous fraction by the help of Column Chromatography. MTT assay was used to evaluate the reduction of viability of the cancer cell lines. Cell viability was inhibited by crude extract of Digera muricata in a dose dependent manner ranging from 25μg/ml to 250μg/ml. Apoptosis assays using nucleic acid stains namely PI exclusion assay and Hoestch/PI assay were performed by the help of fluorescence microscopy. Morphological analysis was done by calculation of Apoptotic ratio and Percentage apoptosis. Our results suggests that methanolic and aqueous fraction of the extract of Digera muricata can be good source of cytotoxic compounds.

Key words: 3-(4,5-dimethylthiazol-2yl)-2,4 diphenyltetrazolium bromide assay, A549 cell line, cytotoxic, Digera muricata, HeLa cell line.

 

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