Estrogenic Activity of Bauhinia racemosa extract in Female albino rats: An Investigational Study

Abstract:

Objective: To evaluate the effect of petroleum ether, ethanol and water extract of Bauhinia racemosa in female albino rats. Methods: Plant extracts were tested for their estrogenic activity by using ovariectomised method at two dose level: 200 mg/kg and 400mg/kg respectively. Further, Plant extracts and Standard drug Ethinyl estradiol (1µg/kg) combination were tested for synergistic estrogenic activity. Results: In-vivo investigation revealed that ethanol extract at dose of 200 mg/kg and 400 mg/kg shows significance increase 325.23±5.23, 328.84±4.56 and 3.252±0.47, 3.288±0.27 in uterine wet weight and uterine weight ratio respectively in female albino rat as compared to control and petroleum ether and water extracts. Simultaneous administration of standard drug Ethinyl estradiol (1µg/kg) with ethanol extract at dose of 200 mg/kg and 400 mg/ kg potentiates (327.36 ± 5.78, 330.95 ± 6.21 and 3.273 ± 0.64, 3.309 ± 0.49 uterine wet weight and uterine weight ratio respectively in female albino rat) the estrogenic activity as compared to individual administration of ethanol extract as a synergistic effect. Conclusion: It was observed that ethanol extract of Bauhinia racemosa produced significance estrogenic activity.

Key words: Bauhinia racemosa, estrogenic activity, Ethinyl estradiol, ovariectomy.