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Published on:3rd Sept, 2014
Pharmacognosy Journal, 2014; 6(6):10-14
Original Article | doi:10.5530/pj.2014.6.3

High-Performance Thin-Layer Chromatography Fingerprinting of Ethnopharmacological Important Seeds of Wrightia tinctoria

Authors and affiliation (s):

Rajani Srivastava1*, Alok Mukerjee2, Amita Verma1

1Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences-Deemed University, Allahabad, Uttar Pradesh, India,

2United Institute of Pharmacy, Allahabad, Uttar Pradesh, India


Introduction: Wrightia tinctoria R. Br. belongs to family Apocynaceae commonly called as sweet Indrajao, Pala indigo plant, Dyer’s Oleander. “Jaundice curative tree” in south India. Materials and Methods: A method has been developed for different extracts of W. tinctoria for high-performance thin-layer chromatography (HPTLC) fingerprinting analysis for identification and quantification of the marker compound. Result: The satisfactory resolution was obtained in the solvent system toluene:ethyl acetate v/v (8:2) for petroleum ether extract, toluene:ethyl acetate v/v (7:3) for chloroform extract and toluene:ethyl acetate:formic acid v/v (7:3:0.1) for alcoholic extract. Conclusion: The HPTLC fingerprinting profile developed for different extracts of W. tinctoria will help in proper identification and quantification of the marker compound.

Key words: High-performance thin-layer chromatography fingerprinting, Wrightia tinctoria, petether extract, chloroform extract, alcoholic extract.


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