Evaluation of antioxidant capacity and acetylcholinesterase inhibitory activity of field-grown plants and in vitro plantlets of Enicostemma littorale

Abstract:

Background: Enicostemma littorale is an ethno-medicinally important plant belonging to Gentianaceae family. Objective: The main aim of this study was to establish tissue culture of E. littorale and compare the antioxidant capacities and acetylcholinesterase inhibitory (AChEi) properties of in vitro and fi eld-grown E. littorale plants. Materials and Methods: Antioxidant capacities for various E. littorale extracts were studied in terms of 1,1-diphenyl-2-picrylhydrazyl and 2,2′-azinobis-3 ethylbenzothiazoline-6-sulfonic acid free-radical scavenging along with ferric reducing antioxidant power and total phenolic content was also determined. Evaluation of AChEi properties was also compared between fi eldgrown and in vitro E. littorale extracts. Results: In vitro plantlets of E. littorale was established in Murashige and Skoog (MS) medium with three different hormonal combinations, viz. naphthalene acetic acid (1 mg/l) and 6-benzylaminopurine (BAP)(0.2 mg/l) or N1B0.2, BAP (1 mg/l) and kinetin (0.1 mg/l) or B₁K_0.1 and indole butyric acid (IBA) (1 mg/l) or IBA1. A lcoholic extracts of shoots developed in MS medium supplemented with B₁K_0.1 showed highest antioxidant potential than other extracts. Alcoholic extracts of plantlets grown in MS medium supplemented with IBA₁ showed both antioxidant and AChEi potential equivalently to fi eld-grown extracts. Conclusion: In vitro plantlets grown in MS medium half strength supplemented with IBA₁ can be considered as most suitable for rapid proliferation because it offers advantage of equivalent potential of bioactivity in terms of antioxidant capacity and AChEi when compared to fi eld-grown plants. Further studies can lead to better utilization of therapeutic potential of E. littorale.

Key words: Acetylcholinesterase inhibition, antioxidant, Enicostemma littorale, secoiridoids