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Published on:27th Nov, 2014
Pharmacognosy Journal, 2015; 7(1):32-36
Original Article | doi:10.5530/pj.2015.1.3

Evaluation of cytotoxic, DNA protecting and LPS induced MMP-9 down regulation activities of Plectranthus amboinicus (Lour) Spreng. essential oil.

Authors and affiliation (s):

Ramaraj Thirugnanasampandana,*, Gunasekar Ramyaa, Madhusudhanan Gogulramnatha, Rajarajeswaran Jayakumarb, M.S. Kanthimathib

aLaboratory of Plant Tissue Culture and Molecular Bioprospection, PG and Research Department of Biotechnology, Kongunadu Arts and Science College, GN Mills, Coimbatore, Tamil Nadu, India

bDepartment of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.


Introduction: Plectranthus amboinicus (Lour) Spreng is a known medicinal plant used in Siddha and Ayurveda medicines in India. It has enormous medicinal potential to treat various diseases. Methods: The present study focused on the use of essential oil obtained from the leaves of Plectranthus amboinicus to test cytotoxicity against breast (MCF-7) and colorectal (HT-29) cancer cell lines, to protect DNA from H2O2 induced genotoxicity through comet assay and to treat inflammation in lipopolysaccharide (LPS) induced over expression of matrix metalloproteinase-9 (MMP-9) in human peripheral blood mononuclear cells (PBMCs) by gelatin zymogram and inhibition at transcriptional level confirmed using RT-PCR (reverse transcriptase polymerase chain reaction). Results: Cytotoxicity of essential oil against MCF-7 and HT-29 cancer cell lines revealed the IC50 values of 53 ± 0.01 and 87 ± 0.01μg/mL respectively. At 200 μg/mL essential oil protected against 75% of DNA damage in 3T3-L1 fibroblast cells. Essential oil showed significant reduction in production of MMP-9 in a concentration dependent manner. Conclusion: Overall, the results showed that essential oil of P. amboinicus is a potent bioactive substance and it could be used in herbal medicine preparations.
Key words: Essential oil , Cytotoxicity , Antigenotoxicity, Zymogram, RT- PCR.


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