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Published on:27th Dec, 2014
Pharmacognosy Journal, 2015; 7(2):89-96
Original Article | doi:10.5530/pj.2015.2.2

Effects of standardized stem bark extract of Mangifera indica L. in Wister rats with 2,4-dinitrophenylhydrazine-induced haemolytic anaemia


Authors and affiliation (s):

Hassan Lawal Sani1, Ibrahim Malami*1, Sanusi Wara Hassan2, Alhassan Muhammad Alhassan3, Mshelia Emmanuel Halilu1, Aliyu Muhammad4

1Department of Pharmcognosy and Ethnopharmacy, Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria

2Department of Biochemistry, Faculty of Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria

3Department of Pharmaceutical Medicinal and Chemistry, Faculty of Pharmaceutical Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria

4Department of Biochemistry, Faculty of Sciences, Ahmadu Bello University, Zaria, Nigeria

Abstract:

Background: The aqueous decoction of the stem back of Mangifera indica L. has been traditionally used for the treatment of various illnesses among them includes anaemia. Aims: The aim of this study was to investigate the anti-anaemic properties of the standardized stem bark extract of M. indica in animals with 2,4-dinitrophenylhydrazine-induced haemolytic anaemia. Methods and Material: An in vivo animal model was used in this experiment. 2,4-dinitrophenylhydrazine was used to induce haemolysis and treatment was done with three different concentrations (25, 50, and 100 mg/kg b.wt) of the plant extract. Astifer® was used as a positive control. Haematological parameters such as PCV, HGb concentration, and TLC were performed to determine the present of phytoconstituents within the crude extract. Results: PCV and HGb concentration was increased significantly (p<0.001) at a dose of 50 and 100 mg/kg b.wt respectively while no significant (p>0.05) effect was observed at a dose of 25 mg/kg b.wt. TLC was decreased significantly (p<0.001) at a dose 100 mg/kg b.wt when while no significant (p>0.05) effect was observed at a dose of 25 and 50 mg/kg b.wt respectively. GC-MS analysis revealed the presence of 15 compounds among viz: 2,2-Dimethoxybutane, N-Acetyl-Alpha-D-glucosamine, 1,2-Benzenediol, Phenol, 2,4-bis(1,1-dimethylethyl)-, Vitamin E, Pentadecanoic acid, 13-methyl-, methyl ester, 2-Ethylacridine, Benzofuran-6-ol-3-one, 2-(4ethoxycarbonyl)benzylidene-, 9-Octadecanoic acid, (E)-, 2,4,6-Cycloheptatrien-1-one, 3,5-bis-trimethylsilyl-, and Benzo[h]quinoline,2,4-dimethyl-. Conclusion: The results of our present finding suggest the significant anti-anaemic properties of standardized stem bark extract of Mangefera indica L. This finding highlights the potentials of the extract M. indica in the treatment of haemolytic anaemia.

Key words: 2,4-dinitrophenylhydrazine, Anaemia, GC-MS analysis, Haemolysis Mangifera indica L.

 

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