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Published on:8th Jan, 2015
Pharmacognosy Journal , 2015; 7(2):136-146
Original Article | doi:10.5530/pj.2015.2.8

Phytochemical analysis and in vitro antioxidant, antimicrobial, anti-inflammatory and cytotoxicity activities of wood rotting fungi, Trametes ochracea


Authors and affiliation (s):

Govindappa Melappa1*, Ara Roshan1, Chanduri Nithi1, Thouseef Syed Mohummed1, Channabasava1 Chandrappa Chinna Poojari and Yerappa Lakshmikanth Ramachandra2

1Department of Biotechnology, Shridevi Institute of Engineering & Technology, Sira Road, Tumkur-572 106, Karnataka, India

2Department of P.G. Studies and Research in Biotechnology & Bioinformatics, Kuvempu University, Jnana Sahyadri, Shankaraghatta Shimoga, Karnataka -577 451, India.

Abstract:

Objective: The present research was aimed to identify the metabolites in the methaolic and hexane extract of Trametes ochracea and evaluated these extracts to know their in vitro biological activities. Materials and methods Two solvent extracts of T. ochracea was subjected to phytochemical analysis. The two solvent extracts were subjected to in vitro biological activity viz., antioxidant, anti-inflammatory and cytoxicity assays. Results: The methanol extract yielded important phytochemicals viz., saponins, flavonoids, alkaloids, steroids, phenols and tannins compared to hexane. The methanolic extract has shown strong antioxidant activity in all tested methods: The methanol extract was effectively inhibited the heat induced hemolysis, antilipoxygenase activity and also stabilized the membrane, avoided the membrane denaturation, proteinase and xanthine oxidase inhibition.The onion root meristametic cells were inhibited due to toxicity of methanol extract by possessing various cellular abnormalities in various stages of actively growing cells. The yeast cells were dead due to toxicity of methanol extract by possessing cell necrosis and also fragmented the cell DNA. Conclusions: The obtained results clearly indicates that Trametes ochracea methanol extract is having potent phytochemicals, which plays important role in antioxidant, anti-inflammatory, cytotoxicity assays. The further research is needed to identify the exact mechanism is by action of one or combination of active phytochemicals.

Key words: Trametes ochracea, Phytochemicals, Antioxidant, Anti-inflammatory, Cytotoxicity.

 

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