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Published on:16th Jan, 2015
Pharmacognosy Journal, 2015; 7(3):147-151
Original Article | doi:10.5530/pj.2015.3.1

Inhibition on Urease and Thermal Induced Protein Denaturation of commonly used Antiulcer Herbal Products. Study based on in-vitro assays

Authors and affiliation (s):

Haroon Khan*1, Murad Ali Khan2, Abdul Rauf3, Ashhad Haleemi4, Shivkanya Fuloria5, Neeraj Kumar Fuloria5

1Department of Pharmacy, Abdul Wali Khan University Mardan 23200, Pakistan

2Department of Chemistry, Kohat University of Science and Technology, Kohat, Pakistan

3Institute of Chemical Sciences, University of Peshawar, Peshawar -25120, Pakistan

4Department of Pharmacy, University of Peshawar, Peshawar -25120, Pakistan

5Department of Pharmcy, AIMST University,Semeling Campus, Bedong, Kedah Darul Aman-08100, Malyasia


Background: In-vitro urease inhibitory and thermal induced protein denaturation inhibitory activitieswasperformed for two commonly used herbal productsEndemali and Akseer ULCER in the treatment of ulcers.Objectives: to evaluate the antiulcer potential of two commonly used herbal products, Endemali, Akseer ULCER. Material and Method: In urease inhibitory assay , enzyme solution, extract, diferentregaents added and absorbance was measured at 630 nm (50 min, pH 8.2) and thiourea used as standard. In protein denaturation assay the egg albumin was mixed with different concentration of test compounds, buffer absorbance was measured. Aspirin was used as standard. Results: The Endemalihad a profound effect on the urease activity in a concentration dependent manner with EC50 valueof 0.468 mg/ml. The Akseer ULCER antagonized the urease activity markedly with EC50 value of 0.374 mg/ml.These tested herbal products caused marked inhibition of thermal induced protein denaturation in a concentration dependent manner. The potency in the form of EC50 for Endemali, Akseer ULCER was measured as 323, 337 µg/mlrespectively. Conclusion: In short, the tested herbal drug showed strong inhibition on urease activity and inhibition on thermal induced protein denaturation thus our study validated their uses in the treatment of ulcers.

Key words: Akseer ULCER, Endemali, Antiurease activity, Thermal induced protein denaturation.


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