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Published on:29th Apr, 2015
Pharmacognosy Journal, 2015; 7(4):249-253
Original Article | doi:10.5530/pj.2015.4.7

GC-MS Analysis of Phytocomponents in, Pet Ether Fraction of Wrightia tinctoria Seed.

Authors and affiliation (s):

Rajani Srivastava*1, Alok Mukerjee2 and Amita Verma1

1Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences-Deemed to-be- University, Allahabad, INDIA

2United Institute of Pharmacy, Allahabad, Utter Pradesh, INDIA.


Introduction: Wrightia tinctoria R.Br. (Family: Apocynaceae) commonly called “Indrajau” is well known in Indian traditional system for its traditional uses. Materials and Methods: The present investigation was carried out to determine the possible bioactive components of plant seed ethanolic extract, pet ether fraction using GC-MS analysis. 22 components were identified from pet ether fraction obtained from elution of ethanolic extract packed in silica column. Results: The prevailing compounds from fraction F6to F9 were [1,1'-Bicyclopropyl]-2-octanoic acid, 2'-hexyl-, methyl ester (21.39%) , Trilinolein (7.74%), 2-Myristynoyl pantetheine (18.07%), 9-Octadecen-12-ynoic acid, methyl ester (4.46%), 1Hexadecanol,2-methyl (3.77%), Cyclopropane tetradecanoic acid, 2-octyl-, methyl ester (2.36%), 1b, 4a-Epoxy-2H-cyclopenta [3,4] cyclopropa [8,9]cycloundec [1,2-b]oxiren-5 (6H)-one, 7-(acetyloxy) decahydro-2,9,10-trihydroxy-3,6,8,8,10a-pentamethyl (38.91%), Geranyl isovalerate (23.58%), cis-13-Octadecenoic acid (5.91%), Quassin (3.82%), cis-10-Heptadecenoic acid (3.08%), 9,12,15-Octadecatrienoic acid 2-phenyl-1, 3-dioxan-5-yl ester (31.50%), 9,12,15-Octadecatrienoic acid, (Z,Z,Z)-2,3-dihydroxypropyl ester (14.35%), Cyclopropanebutanoic acid, 2-[ [2-[ [2- [(2-pentylcyclopropyl) methyl] cyclopropyl] methyl] cyclopropyl] methyl]-, methyl ester (10.13%), 6,9,12,15-Docosatetraenoic acid, methyl ester (3.39%), 9,12-Octadecadienoic acid, (2-phenyl-1,3-dioxolan-4-yl) methyl ester, trans-( 2.73%), 9,12-Octadecadienoic acid, (2-phenyl-1,3-dioxolan-4-yl) methyl ester, cis-(4.34%), Ursodeoxycholic acid (7.14%), Bufa-20,22-dienolide, 3-(acetyloxy)-14,15-epoxy-16-hydroxy-, (3á,5á,15á,16á)-(4.75%), 5H-Cyclopropa [3,4] benz [1,2-e]azulen-5-one, 9a (acetyloxy)-1,1a,1b,4,4a,7a,7b,8,9,9a-de cahydro-4a,7b,9-trihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-,[1aR-(1aà,1bá,4aá,7aà,7bà,8à,9á,9aà)]-(6.59%), Docosahexaenoic acid, 1,2,3-propanetriyl ester (10.86%), Olean-12-ene-3,15,16,21,22,28-hexol, (3á,15à,16à,21á,22à)-( 4.40%) found as the major components. Conclusion: It could be concluded that, Wrightia tinctoria contains various bioactive compounds. So it is recommended as a plant of phytopharmaceutical importance.

Key words: Bioactive components, Ethanolic extract, GC-MS, Indrajau, Wrightia tinctoria.


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