Home | Articles
Published on:29th Apr, 2015
Pharmacognosy Journal, 2015; 7(4):249-253
Original Article | doi:10.5530/pj.2015.4.7

GC-MS Analysis of Phytocomponents in, Pet Ether Fraction of Wrightia tinctoria Seed.


Authors and affiliation (s):

Rajani Srivastava*1, Alok Mukerjee2 and Amita Verma1

1Department of Pharmaceutical Sciences, Faculty of Health Sciences, Sam Higginbottom Institute of Agriculture, Technology and Sciences-Deemed to-be- University, Allahabad, INDIA

2United Institute of Pharmacy, Allahabad, Utter Pradesh, INDIA.

Abstract:

Introduction: Wrightia tinctoria R.Br. (Family: Apocynaceae) commonly called “Indrajau” is well known in Indian traditional system for its traditional uses. Materials and Methods: The present investigation was carried out to determine the possible bioactive components of plant seed ethanolic extract, pet ether fraction using GC-MS analysis. 22 components were identified from pet ether fraction obtained from elution of ethanolic extract packed in silica column. Results: The prevailing compounds from fraction F6to F9 were [1,1'-Bicyclopropyl]-2-octanoic acid, 2'-hexyl-, methyl ester (21.39%) , Trilinolein (7.74%), 2-Myristynoyl pantetheine (18.07%), 9-Octadecen-12-ynoic acid, methyl ester (4.46%), 1Hexadecanol,2-methyl (3.77%), Cyclopropane tetradecanoic acid, 2-octyl-, methyl ester (2.36%), 1b, 4a-Epoxy-2H-cyclopenta [3,4] cyclopropa [8,9]cycloundec [1,2-b]oxiren-5 (6H)-one, 7-(acetyloxy) decahydro-2,9,10-trihydroxy-3,6,8,8,10a-pentamethyl (38.91%), Geranyl isovalerate (23.58%), cis-13-Octadecenoic acid (5.91%), Quassin (3.82%), cis-10-Heptadecenoic acid (3.08%), 9,12,15-Octadecatrienoic acid 2-phenyl-1, 3-dioxan-5-yl ester (31.50%), 9,12,15-Octadecatrienoic acid, (Z,Z,Z)-2,3-dihydroxypropyl ester (14.35%), Cyclopropanebutanoic acid, 2-[ [2-[ [2- [(2-pentylcyclopropyl) methyl] cyclopropyl] methyl] cyclopropyl] methyl]-, methyl ester (10.13%), 6,9,12,15-Docosatetraenoic acid, methyl ester (3.39%), 9,12-Octadecadienoic acid, (2-phenyl-1,3-dioxolan-4-yl) methyl ester, trans-( 2.73%), 9,12-Octadecadienoic acid, (2-phenyl-1,3-dioxolan-4-yl) methyl ester, cis-(4.34%), Ursodeoxycholic acid (7.14%), Bufa-20,22-dienolide, 3-(acetyloxy)-14,15-epoxy-16-hydroxy-, (3á,5á,15á,16á)-(4.75%), 5H-Cyclopropa [3,4] benz [1,2-e]azulen-5-one, 9a (acetyloxy)-1,1a,1b,4,4a,7a,7b,8,9,9a-de cahydro-4a,7b,9-trihydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-,[1aR-(1aà,1bá,4aá,7aà,7bà,8à,9á,9aà)]-(6.59%), Docosahexaenoic acid, 1,2,3-propanetriyl ester (10.86%), Olean-12-ene-3,15,16,21,22,28-hexol, (3á,15à,16à,21á,22à)-( 4.40%) found as the major components. Conclusion: It could be concluded that, Wrightia tinctoria contains various bioactive compounds. So it is recommended as a plant of phytopharmaceutical importance.

Key words: Bioactive components, Ethanolic extract, GC-MS, Indrajau, Wrightia tinctoria.

 

Articles in PDF, ePUB and Full text are attached to this page. In order to download, print or access these formats you must be logged in.
CAPTCHA
This question is for testing whether you are a human visitor and to prevent automated spam submissions.
13 + 2 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.




 

Article Links