Home | Articles
Published on:18th Feb,2014
Pharmacognosy Journal, 2014; 6(2):124-130
Original Article | doi:10.5530/pj.2014.2.8

Pharmacokinetic study of phyllanthin and hypophyllanthin after oral administration to rats

Authors and affiliation (s):

Madhukiran Parvathaneni*,a, Ganga Rao Battua, Ravikumar Jangitia and Keerthana Diyyaa

aUniversity College of Pharmaceutical Sciences, Andhra University, Visakhapatnam 530 003, India.


Objective: The present study was carried out to develop a sensitive and cost effective HPLC method for the determination of bioactive lignans (phyllanthin and hypophyllanthin) and its application in a pharmacokinetic study. Methods: Identification of lignan compounds on C–18 column was monitored at a range of 199–400nm using photodiode array detector (PDA) with methanol-water (66:34, v/v) as mobile phase at a flow rate of 1ml/min. Carbamazepine was used as internal standard. Results: From the developed method LOD and LOQ values were found to be 56.14ng/ml and 169.99ng/ml for phyllanthin, and 56.04ng/ml and 169.82ng/ml for hypophyllanthin. The validated RP–HPLC method herein was applied for pharmacokinetic studies and Cmax (ng/ml) values for administered three oral doses (2.5, 5 and 10mg/kg) of phyllanthin and hypophyllanthin were 0.28±0.06, 0.53±0.16, 0.98±0.22 and 0.68±0.76, 1.35±0.23, 2.45±0.33, respectively. Conclusion: In conclusion, developed HPLC–PDA method effectively determined the phyllanthin and hypophyllanthin in various solvent and plasma samples. This method was successfully applied in conducting their oral pharmacokinetic studies.

Key words: Phyllanthus amarus, phyllanthin, hypophyllanthin, HPLC–PDA, pharmacokinetics.


Articles in PDF, ePUB and Full text are attached to this page. In order to download, print or access these formats you must be logged in.


Article Links