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Pharmacognosy Journal, 2013; 5(4):156-162
Original Article | doi:10.1016/j.phcgj.2013.07.005

Determination of ursolic acid in fractionated leaf extracts of Ocimum gratissimum Linn and in developed herbal hepatoprotective tablet by HPTLC

Authors and affiliation (s):

Arti Guptaa,*, Navin R. Shethb, Sonia Pandeya, Dinesh R. Shaha, Jitendra S. Yadavc

aDepartment of Pharmacognosy, Maliba Pharmacy College, Bardoli, Gujarat 394350, India

bDepartment of Pharmacognosy, Saurashtra University, Rajkot, Gujarat 360005, India

cDepartment of Pharmaceutics, Vidya Bharati Trust College of Pharmacy, Umrakh, Gujarat 394345, India


Background: Ursolic acid was determined in dichloromethane and ethyl acetate fractions of methanolic extract of Ocimum gratissimum and in developed herbal hepatoprotective tablet by HPTLC method. Methods: Hepatoprotective polyherbal formulationwas prepared using five fractions of three plant extracts namely O. gratissimum, Butea monosperma and Bauhinia variegata. Among these three plants O. gratissimum contains ursolic acid. Chromatographic separationwas performed on silica gel HPTLC plates with petroleum ether:ethyl acetate:acetone (8.2:1.8:0.1, v/v/v) as mobile phase. After drying, the plates were sprayed with 10% (v/v) ethanolic solution of sulfuric acid and heated to 120 C for 3 min. Quantificationwas performed in absorbance/transmittance mode at a wavelength of 530 nm using a computer-controlled densitometer. Results: The presented method was validated for linearity 400e1200 (ng/spot), intraday precision % C.V. (0.58e1.97), and interday precision % C.V. (1.46e2.22). Correlation coefficient (r2 = 0.9960), detection limits as well as recovery values (97.5%e98.22%) were found to be satisfactory. Conclusion: A good correlation was obtained among the standard, samples of polyherbal formulation and fractionated extract of O. gratissimum using HPTLC method.

Key words: Ursolic acid,HPTLC,Ocimum gratissimum,Herbal formulation.


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