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Pharmacognosy Journal, 2013; 5(6):275-280
Original Article | doi:10.1016/j.phcgj.2013.09.003

Evaluation of antioxidant and antimicrobial activities of the phenolic composition of Algerian Arbutus unedo L. roots


Authors and affiliation (s):

Nassim Djaboua,b,*, Mohamed El Amine Diba, Hocine Allalia, Andreas Benderbc, Mohammad A. Kamald, Said Ghalema, Boufeldja Tabtia

aLaboratoire des Substances Naturelles & Bioactives (LASNABIO), Département de Chimie, Faculté des Sciences, Université de Tlemcen, 13000 Tlemcen, Algeria

bLaboratoire Chimie des Produits Naturels (CPN), Campus Grimaldi, Université de Corse, UMR CNRS 6134, 20250 Corte, France

cUnilever Centre for Molecular Informatics, Department of Chemistry, University of Cambridge, CB2 1EW, United Kingdom

dMetabolomics & Enzymology Unit, Fundamental and Applied Biology Group, King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia

Abstract:

Introduction: The composition of phenols and flavonoids present in ethyl acetate and methanolic extracts of Algerian Arbutus unedo L. roots was in this work investigated using a combination of analytical techniques. Methods: Firstly, the total phenolic and flavonoid content was established by an analysis of both extracts using capillary GC-MS and 1H, 13C NMR spectroscopy after fractionation via column chromatography. Secondly, a screening for the antioxidant and antibacterial properties of the compounds identified was performed via 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, as well as ferric-ion reducing power (FRAP) assays. Subsequently, the antibacterial activity of the compounds extracted was determined via disc-diffusion against both Gram-positive and Gram-negative bacteria. Results: It was found that the ethyl acetate extract of Algerian A. unedo L. roots was characterized by higher concentrations of phenolic acids, which also has about 2-3 times better radical scavenger properties than the reference antioxidant used, ascorbic acid. In addition, this extract showed the best antibacterial activity obtained in this study against both Gram-positive bacteria as well as Gram negative bacteria, with minimal inhibition dosage values as low as 25 Ag/disc. Conclusion: To conclude both the antioxidant and the antibacterial properties of the extracts of Algerian A. unedo L. roots could hence be of practical value.

Key words: Arbutus unedo L.,Roots,Catechin,Antioxidant and antimicrobial activity,Phenolic compounds.

 

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