Home | Articles
Published on:
Pharmacognosy Journal, 2013; 5(5):205-210
Original article | doi:10.1016/j.phcgj.2013.08.005

Evaluation of antioxidant, anti-inflammatory and adipocyte differentiation inhibitory potential of Ziziphus mauritiana bark extract

Authors and affiliation (s):

Mandavi S. Deshpande*, Prasad R. Tondare, Shubhangi V. Paygude, Kishori G. Apte, Pradeep B. Parab

APT Research Foundation, Pune 411 041, India


Introduction: Ziziphus mauritiana is a shrubs belonging to family Rhamnaceae and distributed in warm temperature zone from western Africa to India. Earlier, anti-cancer, anti-inflammatory and anti-diabetic activities were attributed to Z. mauritiana. Materials and methods: The antioxidant activity of chloroform, ethanol and aqueous extracts of Z.mauritiana barkwas assessed by ABTS + decolourization assay. The anti-inflammatory activitywas studied inWistar rats by Carrageenan induced paw edema assay.The adipocyte differentiation(in3T3-L1adipocyte cell)andglucose uptake (in CHO-HIRC-mycGLUT4e GFP cells) assay of Z. mauritiana bark aqueous extract was performed. Results: The chloroform, ethanol and aqueous extracts showed good antioxidant activity. The antiinflammatory activity (27.83% reduction in inflammation) was observed with chloroform extract at the concentration of 200 mg/kg body weight when administered orally in rats. The aqueous extract showed reduction in number and size of the oil droplets in 3T3-L1 adipocyte cell cytoplasm which was found to be dose dependent. The aqueous extract stimulated the glucose uptake in the CHO-HIRC-mycGLUT4e GFP cells which was comparable to insulin. Conclusion: The chloroform, ethanol and aqueous extracts showed good antioxidant activity. The antiinflammatory potential was shown by chloroform extract only. The adipocyte differentiation inhibition and glucose uptake in CHO-HIRC-mycGLUT4e GFP cells was induced by aqueous extract.

Key words: ABTS+, decolourization assay, 3T3 adipocytes Catechin, Glut4 translocation, Glucose uptake.


Articles in PDF, ePUB and Full text are attached to this page. In order to download, print or access these formats you must be logged in.


Article Links