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Pharmacognosy Journal, 2013; 5(5):205-210
Original article | doi:10.1016/j.phcgj.2013.08.005

Evaluation of antioxidant, anti-inflammatory and adipocyte differentiation inhibitory potential of Ziziphus mauritiana bark extract


Authors and affiliation (s):

Mandavi S. Deshpande*, Prasad R. Tondare, Shubhangi V. Paygude, Kishori G. Apte, Pradeep B. Parab

APT Research Foundation, Pune 411 041, India

Abstract:

Introduction: Ziziphus mauritiana is a shrubs belonging to family Rhamnaceae and distributed in warm temperature zone from western Africa to India. Earlier, anti-cancer, anti-inflammatory and anti-diabetic activities were attributed to Z. mauritiana. Materials and methods: The antioxidant activity of chloroform, ethanol and aqueous extracts of Z.mauritiana barkwas assessed by ABTS + decolourization assay. The anti-inflammatory activitywas studied inWistar rats by Carrageenan induced paw edema assay.The adipocyte differentiation(in3T3-L1adipocyte cell)andglucose uptake (in CHO-HIRC-mycGLUT4e GFP cells) assay of Z. mauritiana bark aqueous extract was performed. Results: The chloroform, ethanol and aqueous extracts showed good antioxidant activity. The antiinflammatory activity (27.83% reduction in inflammation) was observed with chloroform extract at the concentration of 200 mg/kg body weight when administered orally in rats. The aqueous extract showed reduction in number and size of the oil droplets in 3T3-L1 adipocyte cell cytoplasm which was found to be dose dependent. The aqueous extract stimulated the glucose uptake in the CHO-HIRC-mycGLUT4e GFP cells which was comparable to insulin. Conclusion: The chloroform, ethanol and aqueous extracts showed good antioxidant activity. The antiinflammatory potential was shown by chloroform extract only. The adipocyte differentiation inhibition and glucose uptake in CHO-HIRC-mycGLUT4e GFP cells was induced by aqueous extract.

Key words: ABTS+, decolourization assay, 3T3 adipocytes Catechin, Glut4 translocation, Glucose uptake.

 

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