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Pharmacognosy Journal, 2013; 5(6):281-285
Original Article | doi:10.1016/j.phcgj.2012.12.005

Induction of tetraploid plants of Pogostemon cablin (Blanco) and its quality evaluation


Authors and affiliation (s):

Yu Wu, Ming Li*

Department of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, PR China

Abstract:

Background: To establish and optimize the rapid propagation technology of Pogostemon cablin (Blanco) and to induce polyploidy plants of P. cablin (Blanco). Methods: Shoot tips from in vitro-grown plants were treated with four different concentrations of colchicine solution (0.01%, 0.04%, 0.08%, 0.1%), and shaken (100 rpm) at 25oC for 2, 4, 8 h, respectively. The induced buds were identified by stem-tip chromosome determination and stomatal apparatus observation. Six selected tetraploid lines were transferred to the field, and the leaf characteristics, contents of volatile oil were respectively evaluated to provide evidence of high-yield and good qualities of tetraploid P. cablin (Blanco). Results: Six lines of tetraploid plants were obtained. The optimum concentration of colchicine treatment for tetraploid induction was 0.04% for 2 h. The chromosome number of the tetraploid plantlet was 2n = 4 × = 56. All tetraploid plants showed typical polyploidy characteristics. Three elite tetraploid lines possessed higher productivity of volatile oil than those of the control. Conclusion: Obtained tetraploid lines will be of important genetic and breeding value and can be used for further selection and plant breeding.

Key words: Chromosome determination,Colchicines,Micro propagation,Pogostemon cablin (Blanco),Tetraploid.

 

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