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Pharmacognosy Journal, 2013; 5(6):247-255
Original Article | doi:10.1016/j.phcgj.2013.10.005

Pharmacognostic standardisation of Combretum albidum G. Don leaf; a medicinally important liana

Authors and affiliation (s):

Sreekanth Sreedhara, Unnikrishnan Prakash Kumarb, Thekkum Parambil Girijaa, Azhimala Bhaskaranpillai Remashreea,*

aCentre for Medicinal Plants Research (CMPR), Arya Vaidya Sala, Malappuram 676 503, Kerala, India

bKancor Ingredients Limited, Kancor Road, Angamaly 683 573, Kerala, India


Objective: To establish the standardization parameters for complete pharmacognostic evaluation of leaves of Combretum albidum G. Don, a medicinally important liana. Materials and methods: Macro- and microscopical, physico-chemical evaluations of leaves were investigated and preliminary phytochemical analysis, GCeMS analysis and HPTLC profiles were carried out for qualitative phytochemical evaluation of various extracts of leaves of C. albidum. Results: Macro, micro, powder and scanning Electron Microscopy (SEM) of leaf were examined and recorded the result. Physico-chemical parameters like moisture content (11.02 ± 0.05) % w/w, total ash (13.79 ± 0.05) % w/w and respectively acid insoluble ash (0.48 ± 0.01) % w/w extractive and values of alcohol (13.42 ± 0.01) % w/w and water (20.28 ± 0.02) % w/w and sequential extractive values of sample in petroleum ether (5.14%), ethyl acetate (4.55%) and methanol (11.48%) were also evaluated. Preliminary phytochemical analysis indicated the presence of tannins, flavonoids, triterpenes, glycosides and saponins. Awell resolved HPTLC profileswas recorded in sequential extraction with petroleum ether, ethyl acetate and methanol. GCeMS analysis showed the presence of 18 compounds such as caryophyllene (21.58%), linalool (10.69%), β-phellandrene (8.74%) and phytol (7.02%) were the other major compounds present. Conclusions: The above parameters, which are being reported for the first time, are significant towards establishing the pharmacognostic standards for future identification and authentication of genuine plant material.

Key words: Combretum albidum, Pharmacognostic standardisation, Histochemical study, Physico-chemical evaluation.


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