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Pharmacognosy Journal, 2013; 5(5):238-241
Original article | doi:10.1016/j.phcgj.2013.09.006

Phytochemical analysis, HPTLC finger printing, in vitro antioxidant and cytotoxic activity of Cymodocea serrulata


Authors and affiliation (s):

Nanthakumar Ramalingama,*, Karthikeyan Ramakrishnanb, Chitra Krishnana, Seethalakshmi Sankarc

a Faculty of Pharmacy, Sri Ramachandra University, Porur, Chennai 600116, India

b K.M. College of Pharmacy, Uthangudi, Madurai, Tamilnadu, India

c Department of Pharmacology, SRMC & RI, Porur, Chennai 600116, India

Abstract:

Objective: To investigate the phytochemical analysis, HPTLC finger printing, in vitro antioxidant and cytotoxic activity of the methanolic extract of Cymodocea serrulata (MECS). Methods: Phytochemical analysis was carried out to determine the presence of phenol, triterpenoids, flavones, alkaloids, reducing sugars, glycosides, saponins, quinones, proteins, tannins and anthraquinones. HPTLC finger printing was performed using Camag Linomat-V. Antioxidant potential of MECS was determined by DPPH radical scavenging assay, lipid peroxidation inhibition assay and ascorbic acid estimation. Cytotoxic activity was assessed using MTT assay on HeLa cells. Results: Phytochemical analysis and HPTLC profile of MECS revealed that it possess various phytoconstituents. DPPH radical scavenging assay and lipid peroxidation inhibition assay were tested from 1.5 mg/ ml to 1000 mg/ml. The results of these studies showed that at 1000 mg/ml DPPH assay showed 87.81  0.65 inhibition and lipid peroxidation inhibition assay showed 37.15  1.96 inhibition. In cytotoxicity assay, MECS produced maximum percentage of growth inhibition 40.47% at 100 mg/ml. Conclusion: The results of the present study indicated thatMECS possess antioxidant and cytotoxic activity and could be used as a source of antioxidant in the food industry. However further detailed phytochemical, pharmacological and in vivo studies should be the next step in the identification of active constituents.

Key words: Cymodocea serrulata, Methanolic extract, Phytochemical analysis, HPTLC finger printing, Antioxidant and cytotoxic activity.

 

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