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Published on:3rd August, 2012
Pharmacognosy Journal, 2012; 4(31):05-10
Original Article | doi:10.5530/pj.2012.31.2

HPLC fingerprint of buckwheat from different habitats and varieties


Authors and affiliation (s):

Lianxin Peng1, Shu Wang1, Liang Zou1, Jianglin Zhao1 and Gang Zhao1

1College of Biotechnology Industry, Chengdu University, Chengdu 610106, China

Abstract:

Introduction: Originated from China, buckwheat belongs to Fagopyrum (Polygonaceae) and is widely planted worldwide. The authentication and quality control of buckwheat was difficult in present. For quality control and authentication of buckwheat, a rapid and accurate method should be established. Methods: Fingerprint study of buckwheat collected from different habitats and varieties (Table 1) was carried out in this work. A simple and rapid method was established by high-performance liquid chromatography coupled to a UV/VIS detector. The data was subjected to common chemometrics analyses, including similarity analysis (SA), principal component analysis (PCA) and hierarchical clustering analysis (HCA). Results: The HPLC fingerprint varied among buckwheat samples from different sources. It was clear to distinguish Fagopyrum tataricum (L.) Gaertn and F. esculentum Moench from their chemical features. Conclusions: This method was simple, accurate, and useful for quality controlling and distinguishing buckwheat.

Keywords: buckwheat, chemical fingerprint, chemometrics.

 

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