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Published on:17th May 2010
Pharmacognosy Journal, 2010; 2(9):289–292
original Article | doi:Nill

Evaluation of In vitro Antioxidant Activity of Some Plants of Cachar District, Assam.

Authors and affiliation (s):

Paul S. B.b, Mazumder A. H.ab*, Gogoi H. K.a, Gogoi B. J.a, Chaurasia A Ka, Singh La, Srivastava R. B.c

aDefence Research Laboratory, Post Bag No. 02, Tezpur, Assam, 784 001, India

bDepartment of Chemistry, Assam University, Silchar, Assam, 788 011, India

cDirectorate of Life Sciences , Ministry of Defence, Govt. of India, DRDO HQ, New Delhi, India.


The present study was undertaken to evaluate in vitro antioxidant activity of methanolic extract from leaves of four plants viz. Clerodendron colebrookianum Walp. (Verbinaceae), Gnetum gnemon L. (Gnetaceae), Sarcochlamys pulcherrima (Roxb.) Gaud. (Urticaceae), Garcinia lancifolia (Don) Roxb. (Cluciaceae), from Cachar district, Assam, India. DPPH (1,1-diphenyl 2-picrylhydrazyl) radical scavenging capacity, reducing power assay (RPA) and photochemiluminescence (PCL) assay were used for evaluating in vitro antioxidant activity. Total phenolic content (TPC) was estimated by Folin–Ciocalteu’s method. Sarcochlamys pulcherrima showed highest antioxidant activity (DPPH EC50 9.70±1.51ppm), as compared to C. colebrookianum (121.05±1.09ppm), G. gnemon (255.99±0.82ppm), and G. lancifolia (344.96 ±0.76ppm). Highest activity of S. pulcherrima was also supported by RPA and PCL and highest TPC (0.33 mg gallic acid equivalent/mg of dry extract) amongst the plants, indicated that phenolic compound are mainly responsible for the activity.

Key words: S. pulcherrima, Antioxidant activity, DPPH, PCL, RPA, TPC.


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