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Published on:2nd June 2010
Pharmacognosy Journal, 2010; 2(10):351–356
Original Article | doi:Nill

A Study of Antimicrobial Activity of Few Medicinally Important Herbal Single Drugs Extracted in Ethanol, Methanol and Aqueous Solvents

Authors and affiliation (s):

Ayesha Mateen1*, Parwez Ahmed1, Gupta VC.1, Mushtaq Ahmed 1, Amin Uddin2, Arfin Shamshul2, Janardhan K.3

1Central Research Institute of Unani Medicine, Opp, E.S.I. Hospital, Erragadda, Hyderabad – 500838 (A.P), India.

2Central Council for Research in Unani Medicine, 61-65, Institutional Area, Janakpuri, NewDelhi – 110058.

3Head P.G Department of Biotechnology, A.V.College Hyderabad (A.P), India.


There is a currently worldwide upsurge in the use of herbal preparations and active ingredients of medicinal plant in health care. This is particularly true in the rural areas of Asian countries where herbal medicines are the only choice for treating human ailments. Present study reveals the difference in the antimicrobial activity pattern of Hemidesmus indicus, Smilax china,Ocimum basilicum, Ocimum sanctum, Ocimum cannum, Azadirachta indica A.Juss, Trigonella Foenum graceum and piper cubeba extracted in Ethanol, Methanol and Aqueous solvents against the pathogenic organisms E.coli (ATCC-25922), Staphylococcus aureus (ATCC-25923), Pseudomonas aeruginosa (ATCC-27853),Bacillus subtilis(L10969), Bacillus subtilis (SL5740) and clinically isolated strains Shigella, Klebsiella, Proteus, Salmonella paratyphi, Salmonella typhi and Stapylococcus aureus. Among all the extracts Ethanol shows more activity with a zone of inhibition ranges from 10mm to 22mm. And Aqueous extract shows less inhibition which ranges from 6mm to 11mm.

Key words: Anti-bacterial, Herbal drugs, Extracts


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