Anti-bacterial activity of herbal extracts, EuMiL® and antibiotics against Helicobacter muridarum

Abstract:

Aims The aim of this research was to investigate the anti-bacterial activity of herbal extracts of Asparagus racemosus Wild (AR), Centella asiatica Linn (CA). Convolvulus pluricaulis (CP), Emblica officinalis (EO), Ocimum sanctum (OS) and Withania somnifera Dunal (WS), a polyherbal drug EuMil® and antibiotics -Amoxycillin, Metronidazole, Oxy-tetracycline, Roxithromycin and Tinidazole against Helicobacter muridarum a species of helicobactor present in rodents. Materials and methods H.muridarum was isolated from gastric mucosa of control and experimental swiss albino mice on selective media and identified by standard methods. Anti-bacterial activity was assayed by Kirby-Bauer Cup-well agar diffusion method at 5 mg/ml concentration. Conclusions Treatments of extracts of AR, WS, CP, CA and also EuMil® were found to be anti-bacterial and were inhibitory to H. muridarum. C. pluricaulis whole plant extract, EuMil® and Oxytetracycline showed the highest inhibitory activity against H. muradarium. Significance and Impact of study The study signify the importance of these plants as an alternative anti-ulcer and healing agents.

Key words: Ethanopharmacology, Phytochemicals, Traditional medicine, Helicobacter Sp, peptic ulcer, antiulcer.