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Published on:August
Pharmacognosy Journal, 2010; 2(11):381–385
Research Article | doi:10.1016/S0975-3575(10)80019-7

HPTLC Fingerprinting of different leaf extracts of Tylophora indica (Burm f.) Merill.


Authors and affiliation (s):

Gupta Mayank1*, Singh Mhaveer2, Mukhatr Hayat M.1, Ahmad Sayeed2

1SBS College of pharmacy, Patti, Punjab-143416, India.

2Faculty of Pharmacy, Jamia Hamdard, New Delhi-110062, India.

Abstract:

Tylophora indica is very popularly used for the treatment of asthma based on its traditional use for asthma. Tylophora is perennial climbing plant native to the plains, forests, and hills of southern and eastern India. A method has been developed for different extracts of Tylophora indica for HPTLC fingerprinting analysis for identification and quantification of marker compound. For chloroform extract-Chloroform(90): Methanol (5) : Ethyl acetate (5) v/v, Methanol Extract-Toluene(5): Chloroform(90), Ethyl acetate(5) v/v and for Petroleum ether extract-Hexane(40) : Ethyl acetate (60) v/v. The HPTLC fingerprinting profile developed for different extracts of Tylophora indica will help in proper identification and quantification of marker compound.

Key words: Tylophora indica, HPTLC fingerprinting, TLC, Tylophorine.

 

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