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Published on:11/2010
Pharmacognosy Journal, 2010; 2(17):21-23
Research Article | doi:10.1016/S0975-3575(10)80005-7

High Performance Thin Layer Chromatographic Method for Quantitative Determination of Quercetin in Tender Leaves of Psidium guajava


Authors and affiliation (s):

*Bindu A R, Remya K, Aleykutty N A, Sajan J

Department of Pharmaceutical Sciences, Cheruvandoor campus, Ettumanoor, Kottayam, 686631

Abstract:

A sensitive and reliable densitometric High Performance Thin Layer Chromatography method has been developed for the quantification of quercetin, a flavonoid present in leaves of Psidium guajava. Chromatographic analysis was performed using acetone extract of tender leaves of Psidium guajava on silica gel 60 F 254 TLC plates using the solvent system, toluene: acetone: formic acid (38:10:5). Detection and quantification of quercetin was done by densitometric scanning at 364 nm. The results of linearity range and correlation coefficient (0.98470) show that, within the concentration range indicated, there was a good correlation between peak area and corresponding concentration of quercetin. The proposed HPTLC method provided a good resolution of quercetin from other constituents present in acetone extract of tender leaves of Psidium guajava and can be used for the quantification of quercetin.

Key words: HPTLC, Psidium guajava, Quercetin, Flavonoid.

 

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