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Published on:11/2010
Pharmacognosy Journal, 2010; 2(17):74-80
Research Article | doi:10.1016/S0975-3575(10)80015-X

Reduced Coniferin and Enhanced 6-Methoxypodophyllotoxin Production in Linum flavum Cell Cultures

Authors and affiliation (s):

Elfahmi1*, Komar Ruslan Wirasutisna1, Sieb Batterman2, Albert Koulman2, Oliver Kayser2, Herman J. Woerdenbag2, Wim J. Quax2

1School of Pharmacy, Institut Teknologi Bandung Indonesia (ITB), Jl Ganesha 10, Bandung 40116, Indonesia

2Department of Pharmaceutical Biology, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, the Netherlands


Treatment of cell suspension cultures of Linum flavum L. with Na2EDTA reduced the coniferin and enhanced the 6-methoxypodophyllotoxin (6-MPT) production in a concentration-dependent way, in a range of 0.1–5 mM. On day 14 after treatment with Na2EDTA , an inhibition of the coniferin production up to 88% was found. The maximum enhancement of the 6-MPT production was 400% on day 7 after treatment with 5 mM Na2EDTA . The reduction in coniferin accumulation in the suspension cultures correlated with and inhibition of coniferyl alcohol glucosyltransferase (CAGT ) activity as determined in cell homogenates. On day 14 after treatment with 2 and 5 mM Na2EDTA , the CAGT activity was inhibited up to >89 %. The inhibitory effect of Na2EDTA on CAGT was also shown in a partially purified enzyme preparation. Several metal ions and the elicitors nigeran and salicylic acid had no significant effect on the production of coniferin and 6-MPT.

Key words: coniferin; Linum flavum L.; 6-methoxypodophyllotoxin; Na2EDTA ; CAGT inhibition.


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