HPTLC Fingerprint Profile of Extracts from Gum, Bark and Leaf of Boswellia serrata Linn. in Different Solvents

Abstract:

Introduction: Boswellia serrata Linn. tree is commonly found in India. The therapeutic value of its gum (guggulu) has been known to possess good anti-inflammatory, anti-arthritic, anti-proliferative and analgesic activities. Oleo-gum resins from Boswellia species are used in traditional medicine in India and African countries for the treatment of a variety of diseases. Methods: Chromatographic techniques were used for separation of components from different extracts of plant parts. This study was planned to develop a fingerprint profile of drug extracts from different parts of Boswellia serrata Linn. i.e., bark, gum and leaf in different solvents such as petroleum ether, chloroform and water.Results: A high-performance thin layer chromatography (HPTLC) method for the separation of the active constituents in Boswellia serrata extracts has been developed and TLC of these extracts on silica gel precoated aluminium plates of Merck by automatic TLC applicator and using solvent gradient system was performed. Conclusion: The HPTLC method for routine quality control of present species can be carried out using this method for different extracts of plant parts and serve in standardization of the drug.

Key words: Chromatography, quality control, standardization.