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Published on:02 Aug 2010
Pharmacognosy Journal, 2010; 2(12):456-463
Research Article | doi:Nill

Phytochemical and Antimicrobial Activity of Leaf Extract of Asparagus racemosus Willd


Authors and affiliation (s):

Battu GR Kumar BM

Pharmacognosy and Phytochemistry Division, University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh, India.

Abstract:

The aim of the present study was to explore the leaf extract of Asparagus racemosus Willd. belonging to family Asparagaceae for its antimicrobial activity. The in vitro antimicrobial activity of the leaf extract (ethanol-EE) of Asparagus racemosus and its fractions (hexane- HE and chloroform-CE) were assayed using the agar plate diffusion and nutrient broth dilution methods. Test microorganisms studied were Bacillus pumilis, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus aureus, Aspergillus nigerand Candida albicans. EE (300 mg/ml) inhibited the growth of all the test organisms and the maximum zone of inhibition against gram positive organism is S. aureus (14.3 ± 0.2 mm); against gram negative is E. coli (14.0 mm); and against fungal organism is C. albicans (16.2 ± 0.2 mm). EE and CE showed minimum inhibitory concentration (MIC) of 12.5 and 25 mg/ml respectively against the B. pumilis, S. aureus, E. coli and C. albicans. The minimum bactericidal concentration (MBC) of EE was found to be 12.5 mg/ml, against S. aureus and E . coli where as for CE , the MBC was 50 mg/ml against S. aureus, E. coli, B. pumilis and C. albicans . The EE exhibited antimicrobial activities followed by CE. HE exhibited least antimicrobial activity. The preliminary phytochemical screening of EE, CE and HE revealed the presence of sterols, flavonoids, tannins and carbohydrates, determined by utilizing standard methods of analysis. Preliminary phytochemical screening as well thin layer chromatography of the EE, CE and HE revealed that the leaves of A. racemosus contain flavonoids which might possibly be responsible for the antimicrobial activity of the extracts

Key words : Asparagus racemosus; Asparagaceae; Antimicrobial; MIC; MBC; Flavonoids.

 

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