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Pharmacognosy Journal, 2009; 1(2):126-129
Research Article | doi:Nill

Determination of Rutin Content and Antioxidant Activity of Extracts of Butea monosperma Flowers Extracted Using Various Extraction Methods

Authors and affiliation (s):

Edwin Jarald E*, Nalwaya Narendra, Meena Manish, Jain Anurekha, Edwin Sheeja1

*TIFAC-CORE In Green Pharmacy, B.R. Nahata College of Pharmacy, Mhow-Neemuch Road, Mandsaur – 458001, Madhya Pradesh, India.

1Unijules Life Sciences Ltd. & Associated Companies, Nagpur.


In our present study, we quantified rutin and also determined the in vitro antioxidant potential of aqueous extracts of Butea monosperma flowers prepared using soxhlet, decoction, ultrasonic and maceration methods. Presence of rutin was identified using chemical test and TLC method. Quantification of rutin in extracts was done by HPLC method. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitro blue tetrazolium (NBT) assay method was performed at different concentrations (100–400 μg/ml). All the extracts showed the presence of rutin. The percentage yield of crude extract and the quantity of rutin was found to be more in the extract prepared by soxhlet method than the other extracts. The antioxidant activity of extracts increased in a dose dependent manner. Concentration of soxhlet, decoction, ultrasonic and maceration extract needed for 50% inhibition of DPPH were found to be 52.80, 58.02, 60.86 and 52.62 μg/ml respectively. Similarly concentration needed for superoxide scavenging activity was found to be 543.92, 906.61, 752.44 and 680.04 μg/ml respectively. The results of our study showed that the B. monosperma possess a significant antioxidant activity and the maceration extract showed better antioxidant activity than the other extracts in DPPH model and in NBT model, the extract prepared using soxhlet showed better activity compared to other extracts.

Key words: Antioxidant activity; Butea monosperma; Decoction; Maceration; Soxhlet; Ultrasonic.


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