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Pharmacognosy Journal, 2009; 1(2):138-142
Research Article | doi:Nill

A Rapid High-Performance Liquid Chromatographic Method for Quantitative Analysis of Anti-cancerous Active Components in Gloriosa superba Tubers

Authors and affiliation (s):

Rajagopal Chitra*, Kandhasamy Rajamani

Medicinal Plants Unit, Horticulture College and Research Institute, Tamil Nadu Agricultural University ,Coimbatore - 641003, Tamil Nadu.


Extracts of Gloriosa superba tubers with promising anti-cancerous capacity, used in traditional medicine, have been analyzed by high-performance liquid chromatography (HPLC) coupled with diode-array detection (DAD). Two kinds of amino alkaloids were successfully separated and quantified in tubers from eighteen accessions. The colchicine and colchicoside content of tubers from different accessions varied greatly. The colchicine content was found to be at a higher level (0.70 per cent) in the accession ‘Viralimalai cultivated’, whereas the accession ‘Sathyamangalam wild’ recorded the highest colchicoside (6.62 per cent) content.

Key words: HPLC-UV, Gloriosa superba tubers, colchicine and colchicoside, anti-cancerous compounds, Gloriosa accessions.


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