Free Radicals and Antioxidants, 2014; 4(2):8-12
Original Article | doi:10.5530/fra.2014.2.2
Phenolic Compounds From Kenaf (Hibiscus Cannabinus L.) Seeds by Ultrasound-Assisted Extraction
Introduction: There is great interest in the replacement of synthetic antioxidants with natural sources, especially from plant materials. Kenaf seed potentially contained phenolic compounds. Method: Kenaf seed extract was extracted by ultrasonic-assisted extraction. Then, the purification was carried out by using silica gel column chromatography. Lastly, the phenolic compounds were determined by high performance liquid chromatography (HPLC). Result: There were 11 phenolic acids identified from the purified kenaf seed extracts, which were identified as sinapic acid (3563.67 mg/100 g), ferulic acid (2085.55 mg/100 g), catechin hydrate (1189.02 mg/100 g), tannic acid (1032.24 mg/100 g), naringin (385.75 mg/100 g), 4-hydroxybenzoic acid (382.51 mg/100 g), gallic acid (380.18 mg/100 g), 4-hydroxybenzaldehyde (372.00 mg/100 g), protocatehuic acid (207.04 mg/100 g), syringic acid (189.13 mg/100 g) and cinnamic acid (85.51 mg/ 100 g). Conclusion: The kenaf seed extract was found to exhibit an antioxidant capability, and it is potentially suitable to be used as an alternative source of antioxidants
Key words: Phenolic, Kenaf, Chromatography, Antioxidant, Purification.
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