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Published on:9th July 2014
Free Radicals and Antioxidants, 2014; 4(2):8-12
Original Article | doi:10.5530/fra.2014.2.2

Phenolic Compounds From Kenaf (Hibiscus Cannabinus L.) Seeds by Ultrasound-Assisted Extraction

Authors and affiliation (s):

Yu Hua Wong1, Joo Sheng Beh1, Chin Ping Tan2, Kamariah Long3, Kar Lin Nyam1*

1Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, 56000 Kuala Lumpur, Malaysia,

2Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia,

3Malaysian Agricultural Research & Development Institute, 50774 Kuala Lumpur, Malaysia


Introduction: There is great interest in the replacement of synthetic antioxidants with natural sources, especially from plant materials. Kenaf seed potentially contained phenolic compounds. Method: Kenaf seed extract was extracted by ultrasonic-assisted extraction. Then, the purification was carried out by using silica gel column chromatography. Lastly, the phenolic compounds were determined by high performance liquid chromatography (HPLC). Result: There were 11 phenolic acids identified from the purified kenaf seed extracts, which were identified as sinapic acid (3563.67 mg/100 g), ferulic acid (2085.55 mg/100 g), catechin hydrate (1189.02 mg/100 g), tannic acid (1032.24 mg/100 g), naringin (385.75 mg/100 g), 4-hydroxybenzoic acid (382.51 mg/100 g), gallic acid (380.18 mg/100 g), 4-hydroxybenzaldehyde (372.00 mg/100 g), protocatehuic acid (207.04 mg/100 g), syringic acid (189.13 mg/100 g) and cinnamic acid (85.51 mg/ 100 g). Conclusion: The kenaf seed extract was found to exhibit an antioxidant capability, and it is potentially suitable to be used as an alternative source of antioxidants

Key words: Phenolic, Kenaf, Chromatography, Antioxidant, Purification.


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