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Short Communication | doi:10.1016/j.fra.2013.03.004

Antioxidant activity profiling by spectrophotometric methods of phenolic extract of Prasium majus L

Authors and affiliation (s):

Tarik Md. Chaouche a,*, Farah Haddouchi a, Riadh Ksouri b, Faten Medini b, Imad A. El-Haci a, Zahia Boucherit c, Fatima Zohra Sekkal d, Fawzia Atik-Bekara a

a Laboratory of Natural Products, Department of Biology, Faculty of Sciences, Tlemcen University, Tlemcen, Algeria

b Laboratory of Extremophile Plants, Borj Cedria Biotechnology Centre (CBBC), Hammam-Lif 2050, Tunisia

c Antibiotics Antifungal Laboratory: Physical-Chemistry, Synthesis and Biological Activity, Department of Biology, Faculty of Sciences, Tlemcen University, Tlemcen, Algeria

d Department of Biotechnology, Faculty of Natural Science and Life, Abdelel Hamid Ibn Badiss University, Mostaganem 27000, Algeria.


Introduction: Phytochemicals are extensively found at different levels in many medicinal plants. To investigate the phenolic compound content and in vitro antioxidant activity of phenolic extract from Prasium majus L (Lamiaceae). Methods: The present investigation comprises, estimation of total polyphenol, flavonoid, tannin, in vitro antioxidant assays such as total antioxidant capacity, DPPH, ABTS, b-carotene and ferric reducing power. Results: P. majus exhibited 64.25 mg GAE g1 extract of polyphenol phenol content, and better scavenging activity of DPPH (IC50 ¼ 7.95 mg mL 1), ABTSþ (IC50 ¼ 373.78 mg mL 1), and b-carotene (IC50 ¼ 122.56 mg mL1). Conclusion: Our results clearly demonstrated that phenolic extract P. majus has antioxidant capacity. Therefore is a valuable source of natural antioxidants.

Key words: Prasium majus,Quantification of phenolic classes, Antioxidant activity.


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