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Original Article | doi:10.1016/j.fra.2013.06.001

Spinning, oxidative damage and hemolysis in athletes

Authors and affiliation (s):

Giuseppe Gallo*,

Guglielmo Martino, Annarita Carino Laboratory of Cell Physiology, Department of Biology, Ecology and Earth Sciences, University of Calabria, Rende (CS) 87036, Italy


Background: The aim of this study is to put into evidence and verify the correlation between hemolysis and oxidative damage in subjects who practice spinning. Methods: A total of 12 volunteers, aged 35  5 years were enrolled in the present study (60 min) before and (60 min) after practicing spinning. The measurement of lipid peroxidation products and the hemolysis assays conducted on blood samples of athletic subjects, and also the red blood cell morphological study. Results: The obtained data evidence that there are significant differences at least 60 min after training: in malonyldialdehyde value (0.12  0.05 nmol/ml) and in malonyldialdehyde value with 2,20-azobis (2-amidinopropane) dihydrochloride (0.22  0.05 nmol/ml), in hemolysis data up to both 80 min (oxidative lysis) and 120 min (2,20 azobis (2-amidinopropane) dihydrochloride lysis) confirmed by microscopical analysis. Conclusion: The described data on red blood cell hemolysis, after exercise-induced oxidative damage (malonyldialdehyde), and the degradation kinetic under action of 2,20-azobis (2-amidinopropane) dihydrochloride can be described according to a multistage process with multiple contemporaneous equilibria. The final red blood cell echinocytic form could describe the end product of process. Consequently authors hypothesize a relationship between spinning exercise, plasma membrane oxidation and hemolysis susceptibility.

Key words: AAPH ,Hemolysis ,Oxidative damage ,Spinning, MDA.


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