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Published on:8th April 2014
Pharmacognosy journal, 2014; 6(3):49-54
Original Article | doi:10.5530/pj.2014.3.8

Evaluation of Antioxidant Activity of Five Medicinal Plants in Sri Lanka.

Authors and affiliation (s):

K. N. Mahesh1, M. N. Wickramaratne2 and D. B. M. Wickramaratne1

1Department of Pharmacy, Faculty of Allied Health Sciences University of Peradeniya Sri Lanka

2Department of Physical Sciences and Technology Faculty of Applied Sciences Sabaragamuwa University of Sri Lanka.


This study investigated on the antioxidant properties of five medicinal plants used in Sri Lanka, namely Solanum nigrum, Amaranthus spinosus, Elephantopuss caber, Amorphophallus campanulatus and Canna indica. The cold methanol plant extracts were screened for the antioxidant activity evaluating their 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical in scavenging ability. The total ascorbic acid content of the extracts was also evaluated. The IC50 values of the extracts revealed that Solanum nigrum had the best DPPH scavenging activity with a value of 37.63 ± 0.11μg/ml and was better than that of the standard ascorbic acid. Amorphophallus campanulatus extract gave the highest ascorbic acid content of 143.03 ± 1.97 mg per 100 g of the extract. All five plants extract showed DPPH scavenging activity in the order of Solanum nigrum > Elephantopus scaber > Amorphophallus campanulatus > Canna indica. The plant extracts did not show a direct correlation between the ascorbic acid content to the DPPH scavenging activity. These experimental results reveals that these extracts can be utilized in future as therapeutic agent against free radical induced oxidative stress.

Key words: Antioxidant activities, Ascorbic acid, DPPH, Medicinal Plants.


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