Pharmacognosy Journal, 2011; 3(20):33-37
Original Article | doi:10.5530/pj.2011.20.7
Inhibitory activities of Ficus benghalensis bark against carbohydrate hydrolyzing enzymes - An in vitro study
The present study evaluated the effect of Ficus benghalensis Linn. (Moraceae) stem bark on porcine pancreatic α-amylase, rat intestinal α-glucosidase and sucrase. Further, the effect of heat treatment was also studied. Both untreated and heattreated samples inhibited α-amylase to a significant extent. Further, the aqueous extracts prepared form untreated and heat treated samples exhibited significant inhibition (p ≤ 0.05) of α-glucosidase and sucrase in a dose dependent manner. Heat treatment of the sample increased α-glucosidase and sucrase inhibitory activities at lower concentrations, however no statistical differences were observed at higher concentrations. Consequently, the untreated extracts showed IC50 values of 158 and 193 μg mL–1 for α-glucosidase and sucrase respectively while, the heat-treated extracts showed the IC50 values 77 and 141 μg mL–1 respectively. Further, a significant correlation (p ≤ 0.05; r = 0.698) was observed between α-glucosidase and sucrase inhibitory activities of both untreated and heat-treated extracts. The results clearly demonstrate that inhibition of carbohydrate hydrolyzing enzymes is one of the mechanism through which F. benghalensis bark exerts its hypoglycemic effect in vivo.
Key words: Ficus bengalensis, α-amylase, α-glucosidase, sucrase, diabetes.
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