Pharmacognosy Journal, 2014; 6(6):10-14
Original Article | doi:10.5530/pj.2014.6.3
High-Performance Thin-Layer Chromatography Fingerprinting of Ethnopharmacological Important Seeds of Wrightia tinctoria
Introduction: Wrightia tinctoria R. Br. belongs to family Apocynaceae commonly called as sweet Indrajao, Pala indigo plant, Dyer’s Oleander. “Jaundice curative tree” in south India. Materials and Methods: A method has been developed for different extracts of W. tinctoria for high-performance thin-layer chromatography (HPTLC) fingerprinting analysis for identification and quantification of the marker compound. Result: The satisfactory resolution was obtained in the solvent system toluene:ethyl acetate v/v (8:2) for petroleum ether extract, toluene:ethyl acetate v/v (7:3) for chloroform extract and toluene:ethyl acetate:formic acid v/v (7:3:0.1) for alcoholic extract. Conclusion: The HPTLC fingerprinting profile developed for different extracts of W. tinctoria will help in proper identification and quantification of the marker compound.
Key words: High-performance thin-layer chromatography fingerprinting, Wrightia tinctoria, petether extract, chloroform extract, alcoholic extract.
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