Pharmacognosy Journal, 2013; 5(6):275-280
Original Article | doi:10.1016/j.phcgj.2013.09.003
Evaluation of antioxidant and antimicrobial activities of the phenolic composition of Algerian Arbutus unedo L. roots
Introduction: The composition of phenols and flavonoids present in ethyl acetate and methanolic extracts of Algerian Arbutus unedo L. roots was in this work investigated using a combination of analytical techniques. Methods: Firstly, the total phenolic and flavonoid content was established by an analysis of both extracts using capillary GC-MS and 1H, 13C NMR spectroscopy after fractionation via column chromatography. Secondly, a screening for the antioxidant and antibacterial properties of the compounds identified was performed via 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging, as well as ferric-ion reducing power (FRAP) assays. Subsequently, the antibacterial activity of the compounds extracted was determined via disc-diffusion against both Gram-positive and Gram-negative bacteria. Results: It was found that the ethyl acetate extract of Algerian A. unedo L. roots was characterized by higher concentrations of phenolic acids, which also has about 2-3 times better radical scavenger properties than the reference antioxidant used, ascorbic acid. In addition, this extract showed the best antibacterial activity obtained in this study against both Gram-positive bacteria as well as Gram negative bacteria, with minimal inhibition dosage values as low as 25 Ag/disc. Conclusion: To conclude both the antioxidant and the antibacterial properties of the extracts of Algerian A. unedo L. roots could hence be of practical value.
Key words: Arbutus unedo L.,Roots,Catechin,Antioxidant and antimicrobial activity,Phenolic compounds.
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