Pharmacognosy Journal, 2013; 5(2):46-55
Original Article | doi:10.1016/j.phcgj.2013.02.003
Pharmacognostic specifications of five root species in Ben-Cha-Moon-Yai remedy: Thai traditional medicine remedy
Introduction: Ben-Cha-Moon-Yai remedy is one of the Thai traditional medicines notified in Tumrapaadsard song khor. This remedy has been used as an antipyretic, anti-inflammatory and analgesic drug in Thai traditional medicine practice. Establishment of the pharmacognostic profile of five roots species in Ben-Cha-Moon-Yai remedy will be able to guarantee quality, purity and identification of remedy. Materials and methods: Five root species in Ben-Cha-Moon-Yai remedy were collected from various sources throughout Thailand; then investigated the pharmacognostic specification of each root species following WHO guideline of quality control method for medicinal plant materials. High performance liquid chromatography coupled with photo diode arrays was applied to analyze their fingerprints. Results: Macroscopic study was demonstrated as whole plant drawing; while microscopic study was investigated in powdered drugs and its transverse section for anatomy and histology evaluation. The comparative characteristics and physico-chemical parameters of five root species were revealed for standardization. The representative fingerprints revealed a common peak of each root species and Ben- Cha-Moon-Yai remedy under the same HPLC condition. Conclusion: The results obtained from macroscopic and microscopic inspections, physico-chemical parameters, and developed HPLC fingerprints can be used to standardize five root species in Ben-Cha- Moon-Yai remedy.
Key words: Pharmacognostic specification, Quality control, High performance liquid chromatography/photodiode array detector (HPLCePDA)
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