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In Vitro Antioxidant Activity of Extracts from Fruits of Muntingia calabura Linn. from India
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Published on:10th sep 2010
Pharmacognosy Journal, 2010; 2(14):11-18
Research Article | doi:Nill

In Vitro Antioxidant Activity of Extracts from Fruits of Muntingia calabura Linn. from India


Authors and affiliation (s):

K. Preethi, N. Vijayalakshmi, R. Shamna, J. M. Sasikumar*

1Research and Development Centre, Bharathiar University, Coimbatore – 641046, India.

2*Department of Biotechnology, School of Life Sciences, Karpagam University, Coimbatore – 641 021, India

Abstract:

Introduction: In the present study, hexane, chloroform, ethyl acetate, butanol and methanol extracts of fruits of Muntingia calabura L (Elaeocarpaceae) were examined total phenolics (TP) and in vitro antioxidative capacity. Methods: For the determination of total phenolics (TP) and in vitro antioxidative capacity, established assay methods such as 1, 1 - diphenyl – 2- picryl hydroxyl (DPPH) radical assay, reducing power, ferric ion chelating assay, superoxide anion, and nitric oxide scavenging activity assays were used with reference to synthetic antioxidant butyl hydroxyl toluene (BHT). One way analysis of variance (ANOVA) and Duncan’s Multiple Range Test (DMRT) were carried out. Results: The TP ranged from 1486 ± 0.028 mg GAE /100 of fresh mass (FW) to 358 ± 0.020 mg GAE/100 of fresh mass (FW). The data obtained from the study showed high levels of antioxidant activity of the fruit extracts. Conclusion: From the findings, it was observed that there was a well correlation between antioxidant activity and total phenolic/flavonoid contents. These results may be useful to further analyze wild edible fruits that contain most antioxidant activity in order to identify the active constituents.

Key words: Antioxidant activity, butyl hydroxyl toluene, Elaeocarpaceae, Muntingia calabura L., phenolic content.

 

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